Malaria in a rural area of Sierra Leone. III. Vector ecology and disease transmission

Abstract

Studies were undertaken on the role of Anopheles gambiae and An. funestus in the transmission of malaria in four villages in a high-rainfall, forested area in the Bo district of southern Sierra Leone. Anopheles gambiae s.s., identified chromosomally as the Forest form, was the most important vector, with a mean annual sporozoite rate, based on ELISA, of 7.4%. Anopheles funestus, which was found in considerably lower numbers, was mainly a dry season vector, with an annual sporozoite rate of 11.4%. Despite these relatively high sporozoite rates, vector populations were at a low level, with approximate mean densities of only 1.0 An. gambiae and 0.1 An. funestus resting females per house room, and average biting rates of just 1.1 and 0.1 bites/person/night by these two species, respectively. In the rainy season, biting rates peaked at 9.5 An. gambiae bites/person/night and 1.0 An. funestus bites/person/night. Annual sporozoite inoculation rates by An. gambiae and An. funestus were 0.088 and 0.007 infective bites/person/night, respectively. ELISA showed that both species were highly anthropophagic. Exit-trap collections and outdoors searches showed that An. gambiae exhibited a considerable degree of exophily. Light traps inside houses caught nine anopheline species, whereas pyrethrum spray collections in houses caught only An. gambiae, An. funestus and An. hancocki.