Induction of Fos immunoreactivity in the rat brain after cold-restraint induced gastric lesions and fecal excretion

Abstract

Cold-restraint alters gastrointestinal function through vagal pathways. Immunohistochemical detection of the nuclear phosphoprotein Fos (Fos-IR) was used to map brain neuronal pathways activated by cold exposure for 3 h in fasted rats maintained individually in semi-cylindrical restraining cages. Gastric lesions and fecal pellet output were also monitored. In rats exposed to cold (4 degrees C) restraint for 3 h, numerous Fos-positive nuclei were observed in the dorsal motor nucleus of the vagus, raphe pallidus, locus coeruleus, and paraventricular nucleus of the hypothalamus, and, to a lesser extent, in the raphe obscurus, parapyramidal region, and medullary noradrenergic region, bed nucleus of the stria terminalis and septum. Fecal pellet output was increased by 8 fold and gastric lesions covered 19.5 +/- 1.1% of the corpus mucosa. Rats restrained at room temperature under otherwise same conditions had little or no Fos-positive cells in these brain nuclei, no gastric erosion and a low pellet output (1.3 +/- 0.5 nb/3 h). These data, in addition to previous functional studies, provide anatomic support for the involvement of neurons in the caudal raphe nuclei, dorsal motor nucleus of the vagus and paraventricular nucleus of the hypothalamus in the autonomic and endocrine responses to cold-restraint.