Methyl-methacrylate as an embedding medium in histopathology
- PMID: 79539
- DOI: 10.1111/j.1365-2559.1977.tb01671.x
Methyl-methacrylate as an embedding medium in histopathology
Abstract
Methyl-methacrylate embedding makes it possible to obtain semi-thin sections rich in detail and without tissue shrinkage. The procedure requires considerable time and labour, and great care must be taken to prevent heat damage to the tissue during the exothermic polymerization process. The original method given by Burkhardt (1966) and later modifications are described and discussed, with special attention to the practical problems encountered and their solutions.
Similar articles
-
Plastic embedding in routine histology. I: Preparation of semi-thin sections of undecalcified marrow cores.Histopathology. 1985 Dec;9(12):1263-74. doi: 10.1111/j.1365-2559.1985.tb02809.x. Histopathology. 1985. PMID: 2420695
-
Embedding iliac bone biopsies at low temperature using glycol and methyl methacrylates.Stain Technol. 1983 Sep;58(5):299-308. doi: 10.3109/10520298309066802. Stain Technol. 1983. PMID: 6200963
-
A new histological embedding method by low-temperature polymerisation of methyl methacrylate allowing immuno- and enzyme histochemical studies on semi-thin sections of undecalcified bone marrow biopsies.Histochemistry. 1992 Oct;98(3):145-54. doi: 10.1007/BF00315873. Histochemistry. 1992. PMID: 1280633
-
A simplified technique for low temperature methyl methacrylate embedding.Stain Technol. 1987 May;62(3):155-9. doi: 10.3109/10520298709107986. Stain Technol. 1987. PMID: 3303457
-
New method of embedding the GMA, quetol 523 and methyl methacrylate for light and electron microscopic observation of semi-thin sections.Okajimas Folia Anat Jpn. 1981 May;58(1):55-68. doi: 10.2535/ofaj1936.58.1_55. Okajimas Folia Anat Jpn. 1981. PMID: 7024886 No abstract available.
Cited by
-
Enzyme and immunohistochemistry on undecalcified bone and bone marrow biopsies after embedding in plastic: a new embedding method for routine application.Virchows Arch A Pathol Anat Histopathol. 1992;420(1):17-24. doi: 10.1007/BF01605979. Virchows Arch A Pathol Anat Histopathol. 1992. PMID: 1539447
-
Kinetic studies of bone and mineral metabolism during treatment with (3-amino-1-hydroxypropylidene)-1,1-bisphosphonate (APD) in rats.Calcif Tissue Int. 1980;32(2):145-57. doi: 10.1007/BF02408534. Calcif Tissue Int. 1980. PMID: 6773632
-
Malignant lymphoma of true histiocytic origin: histiocytic sarcoma. A morphological, ultrastructural, immunological, cytochemical and clinical study of 10 cases.Virchows Arch A Pathol Anat Histol. 1981;391(3):249-65. doi: 10.1007/BF00709158. Virchows Arch A Pathol Anat Histol. 1981. PMID: 7281492
-
Enzyme histochemistry on bone marrow sections after embedding in methacrylate at low temperature.Histochemistry. 1981;70(2):95-105. doi: 10.1007/BF00493201. Histochemistry. 1981. PMID: 7216836 No abstract available.
-
gamma-Glutamyl transpeptidase demonstrated in tissue sections embedded in glycol methacrylate resin.Histochem J. 1987 Sep;19(9):476-82. doi: 10.1007/BF01675417. Histochem J. 1987. PMID: 2894370
MeSH terms
Substances
LinkOut - more resources
Full Text Sources