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. 1994 Jul;26(1):45-8.
doi: 10.1007/BF00326303.

Electrophoretic karyotype of Mucor circinelloides

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Electrophoretic karyotype of Mucor circinelloides

A Nagy et al. Curr Genet. 1994 Jul.

Abstract

Contour-clamped homogeneous electric field (CHEF) gel electrophoresis was used to separate chromosomal size DNA molecules of two Mucor circinelloides strains. Electrophoretic karyotypes revealed the presence of eight distinct bands for the M. circinelloides f. lusitanicus strain, and four, presumably multiple, bands for the M. circinelloides f. gryseo-cyanus strain. The approximate sizes of the resolved chromosomal DNA bands ranged from 2.3 to 8.1 Mb, giving estimated genome sizes of 38.7 and 32.6 Mb, respectively. Hybridisation techniques were used to assign the leuA gene to a chromosome.

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