Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Dec;26(3):425-30.

Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent

S Izui, P H Lambert, P A Miescher

Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent

S Izui et al. Clin Exp Immunol. 1976 Dec.

Abstract

A modified 125I-labelled DNA-binding test was developed. By using an anionic detergent, 0-025% sodium dodecyl sulphate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE patients.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 1968 Aug 23;161(3843):806-7 - PubMed
    1. J Exp Med. 1967 Oct 1;126(4):607-24 - PubMed
    1. J Immunol. 1974 Dec;113(6):1921-7 - PubMed
    1. J Exp Med. 1974 Nov 1;140(5):1189-206 - PubMed
    1. J Clin Invest. 1973 Jan;52(1):198-204 - PubMed

LinkOut - more resources