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. 1994 Nov 14;354(3):320-4.
doi: 10.1016/0014-5793(94)01154-0.

Affinity purification of Hydra glutathione binding proteins

S L Bellis  1 , D C Laux, D E Rhoads
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Free article

Affinity purification of Hydra glutathione binding proteins

S L Bellis et al. FEBS Lett. .
Free article

Erratum in

  • FEBS Lett 1995 Mar 27;362(1):98

Abstract

The association of glutathione (GSH) with putative external chemoreceptors elicits feeding behavior in Hydra. In the present study, solubilized membrane proteins were chromatographed on an affinity column of immobilized GSH in order to isolate GSH-binding proteins that may represent the Hydra GSH chemoreceptor. The most abundant of the affinity-purified proteins was a triplet of peptides ranging in molecular weight from 24.5-26 kDa. Antiserum generated against the 24.5-26 kDa triplet peptides inhibited GSH-stimulated feeding behavior by 47%, implicating a role for one or more of these peptides in Hydra chemoreception.

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