Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Oct;85(10):1029-34.
doi: 10.1111/j.1349-7006.1994.tb02901.x.

Gene expression for tumor necrosis factor alpha and its production in gastric cancer patients

M Ohno  1 , M Kato, T Nakamura, Y Saitoh
Affiliations

Gene expression for tumor necrosis factor alpha and its production in gastric cancer patients

M Ohno et al. Jpn J Cancer Res. 1994 Oct.

Abstract

To determine the gene expression for tumor necrosis factor (TNF) alpha and its main site of production in gastric cancer patients, serum levels in the peripheral venous blood of 50 patients and the portal blood from 15 of these 50 patients were measured by enzyme-linked immunosorbent assay (ELISA). TNF gene expression in the peripheral blood mononuclear cells (PBMC) and in the surgically resected tissues was then studied in 16 patients by reverse transcription-polymerase chain reaction (RT-PCR) assay. Whereas TNF mRNA expression was detected in the PBMC from 13 of 16 gastric cancer patients (81.3%), it was detected in only one tumor tissue (6.3%). Preoperatively, TNF was detected in the serum from 13 of 50 patients (26.0%). In the portal blood sampled immediately after laparotomy, TNF was positive in 4 of 15 patients (26.7%). TNF gene expression was much more frequently detected in PBMC than in other resected tissues, and its expression was higher than in the serum. Various clinicopathological factors for gastric cancer were not related to the preoperative detection of TNF in the serum. It appears that TNF is produced mainly in PBMC but not in the cancer regions or the regional lymph nodes of gastric cancer patients. It is suggested that TNF is not always secreted even when TNF mRNA is expressed, and its preoperative production is not related to tumor progression.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. ) Carswell , E. A. , Old , L. J. , Kassel , R. L. , Green , S. , Fiore , N. and Williamson , B.An endotoxin‐induced serum factor that causes necrosis of tumors . Proc. Natl. Acad. Sci. USA , 72 , 3666 – 3670 ( 1975. ). - PMC - PubMed
    1. ) Carswell E. A. , Green , S. , Everson , T. C. , Nathanson , T. , Biedler , J. L. , Helson , L. and Spengler , B. A.Effect of tumor necrosis factor on cultured human melanoma cells . Nature , 258 , 731 – 732 ( 1975. ). - PubMed
    1. ) Hanaoka , K. and Satorai , N.Cytotoxic activity of tumor necrosis factor (TNF) on human cancer cells in vivo . Jpn. J. Exp. Med. , 51 , 191 – 194 ( 1981. ). - PubMed
    1. ) Oliff , A. , Jones , D. , Boyer , M. , Martinez , D. , Kiefer , D. , Vuocolo , G. , Wolfe , A. and Socher , S. H.Tumors secreting human TNF/cachectin induce cachexia in mice . Cell , 50 , 555 – 563 ( 1987. ). - PubMed
    1. ) Krönke , M. , Hensel , G. , Schlúter , C. , Scheurich , P. , Schútze , S. and Pfizenmaier , K.Tumor necrosis factor and lymphotoxin gene expression in human tumor cell lines . Cancer Res. , 48 , 5417 – 5421 ( 1988. ). - PubMed

MeSH terms

LinkOut - more resources