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. 1994 Nov;176(22):6931-5.
doi: 10.1128/jb.176.22.6931-6935.1994.

Identification of distinct domains for signaling and receptor interaction of the sensory rhodopsin I transducer, HtrI

V J Yao  1 , E N Spudich, J L Spudich
Affiliations

Identification of distinct domains for signaling and receptor interaction of the sensory rhodopsin I transducer, HtrI

V J Yao et al. J Bacteriol. 1994 Nov.

Abstract

The phototaxis-deficient mutant of Halobacterium salinarium, Pho81, lacks both sensory rhodopsin I (SR-I) and its putative transducer protein HtrI, according to immunoblotting and spectroscopic criteria. From restriction analysis and selected DNA sequencing, we have determined that the SR-I- HtrI- phenotype results from an insertion of a 520-bp transposable element, ISH2, into the coding region of the SR-I apoprotein gene sopI and deletion of 11 kbp upstream of ISH2 including the first 164 bp of sopI and the entire htrI gene. SR-I and HtrI expression as well as full phototaxis sensitivity are restored by transformation with a halobacterial plasmid carrying the htrI-sopI gene pair and their upstream promoter region. An internal deletion of a portion of htrI encoding the putative methylation and signaling domains of HtrI (253 residues) prevents the restoration of phototaxis, providing further evidence for the role of HtrI as a transducer for SR-I. Analysis of flash-induced photochemical reactions of SR-I over a range of pH shows that the partially deleted HtrI maintains SR-I interactions sites responsible for modulation of the SR-I photocycle.

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References

    1. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1987-91 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11915-9 - PubMed
    1. EMBO J. 1993 Aug;12(8):2999-3005 - PubMed
    1. J Biol Chem. 1993 Aug 5;268(22):16095-7 - PubMed
    1. Biophys J. 1993 Dec;65(6):2578-85 - PubMed

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