Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Nov 17;148(3):287-94.
doi: 10.1007/BF00332903.

A map of the restriction targets in yeast 2 micron plasmid DNA cloned on bacteriophage lambda

J D Beggs, M Guerineau, J F Atkins

A map of the restriction targets in yeast 2 micron plasmid DNA cloned on bacteriophage lambda

J D Beggs et al. Mol Gen Genet. .

Abstract

The 2 micron circular DNA from S. cerevisiae has been cloned on bacteriophage lambda. The two forms of circular DNA which exist in equilibrium due to recombination between inverted repeat sequences were separated as stable clones, and a map of targets for restriction endonucleases EcoRI, HindIII and HpaI was constructed. The circular DNAs isolated from a particular oligomycin resistant strain and its parent oligomycin snesitive strain were compared by restriction endonuclease analysis, and no difference was detected. The potential uses of cloned 2 micron DNA in determining the possible biological role of these plasmids are considered.

PubMed Disclaimer

References

    1. J Mol Biol. 1975 Nov 5;98(3):551-64 - PubMed
    1. Science. 1967 Jun 2;156(3779):1234-7 - PubMed
    1. Biochemistry. 1973 Jul 31;12(16):3055-63 - PubMed
    1. J Mol Biol. 1970 May 28;50(1):111-27 - PubMed
    1. J Mol Biol. 1960 Dec;2:392-415 - PubMed

LinkOut - more resources