Effect of template size on accumulation of defective interfering RNAs in protoplasts
- PMID: 7966644
- PMCID: PMC237324
- DOI: 10.1128/JVI.68.12.8466-8469.1994
Effect of template size on accumulation of defective interfering RNAs in protoplasts
Abstract
A turnip protoplast system has been used to study the effects of template size and sequence on the replication and/or stability of a small defective interfering (DI) RNA associated with turnip crinkle virus. Our results indicated that as little as a single base difference in the size of the molecule in some regions, rather than the specific sequence, affected the level of DI RNA accumulating in protoplasts.
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