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. 1976 Nov 24;149(1):111-4.
doi: 10.1007/BF00275965.

Temperature-sensitive mutants of the slime mould Physarum polycephalum. I. Mutants of the amoebal phase

A E Wheals, W D Grant, B M Jockusch

Temperature-sensitive mutants of the slime mould Physarum polycephalum. I. Mutants of the amoebal phase

A E Wheals et al. Mol Gen Genet. .

Abstract

A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in clones. Some of these mutants appear to be cell-cycle stage specific.

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