Role of laminin on prolactin and gonadotrophin release from anterior pituitaries of male rats

Abstract

The effect of rat laminin on the release of prolactin (PRL), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from anterior pituitaries of adult male rats was studied in vitro. Laminin had a bidirectional effect on basal release of PRL, being stimulatory at 10(-12)M and inhibitory at 10(-9) and 10(-8)M. LH release was only stimulated by the 10(-12)M concentration and no changes in FSH release were observed. Laminin did not modify the stimulatory action of thyrotropin-releasing hormone (TRH) nor luteinizing hormone releasing hormone (LHRH), but the response of FSH to LHRH was inhibited with a maximal effect at 10(-10)M. To assess its physiological role, the effect of a rabbit antibody to laminin on the release of PRL, LH and FSH was also studied. IgG anti-laminin at 2 x 10(-7)M had a highly significant stimulatory effect on PRL release after 3 and 5 hr of incubation, as compared to IgG from normal rabbit serum and medium alone used as controls. No changes on the release of LH and FSH were detected. These results suggest that laminin plays a physiologically significant inhibitory role on PRL release at the pituitary level in vitro.