[Isolation and function of platelets. I. Platelet rich plasma. Comparison between 2 methods: gel filtration and albumin density gradient centrifugation. II. A new method using total blood: metrizamide gradient centrifugation]

Abstract

Human platelets separated from platelet rich plasma (PRP) by two different methods: gel filtraton and centrifugation on albumin gradient have been compared for yield, cellular and protein contamination, ultra-structure, platelet populations, shape change, aggregability and functional preservation. A new method for the separation of platelets from total blood on metrizamide gradients has been established. This technique is rapid and easy; it avoids the initial centrifugation of the PRP where about 30% of the platelets are lost and needs of blood 5 to 10 ml of blood.