[Experimental study of the stability of avirulent properties and the immunogenicity of suppressor revertants of streptomycin-dependent salmonella mutants]

Abstract

The authors aimed at the experimental confirmation of avirulent properties of suppressor revertants of streptomycin-dependent salmonella mutants and also at the study of their immunogenicity. Stability of the loss of virulence by the suppressor revertants was established by studying the virulence of the subcultures isolated from them, differing by the genotype. Repeated passage of suppressor revertants of S. typhimurium Rev8, S. enteritidis Rev5, S. pullorum Rev through the biological objects highly sensitive to salmonella infection failed to lead to elevation of the virulence of the cultures under study. Live salmonella vaccines prepared from the suppressor revertants proved to be highly immunogenic in single oral and parenteral immunization of albino mice and chicks. Analogous immunization with killed vaccines was much less effective, streptomycin-dependent vaccines occupying the intermediate position.