Analysis of the extracellular proteases of Trichomonas vaginalis
- PMID: 7971921
- DOI: 10.1007/BF00932372
Analysis of the extracellular proteases of Trichomonas vaginalis
Abstract
We have previously isolated two extracellular cysteine proteases (60 and 30 kDa, respectively) from the cell filtrate of an isolate of Trichomonas vaginalis. In this study the clinical presentation of 12 clinical isolates of T. vaginalis was correlated with their protease activity. All 12 isolates produced a 60-kDa protease as demonstrated by immunoblotting. However, only 5 of 12 isolates produced a 30-kDa protease in the extracellular filtrate. Protease activity did not differentiate between isolates obtained from symptomatic versus asymptomatic women. The 60-kDa protease, which breaks down into a 43- and 23-kDa subunit, was detected by immunoblotting with anti-23-kDa cross-reacting rabbit serum in the vaginal washes of 3/3 women with active T. vaginalis infection, 0/1 woman cured of T. vaginalis and 0/2 control women with vaginal candidiasis. These results suggest that the 60-kDa protease is found in all isolates testes in vitro, is present in active disease and may be important in the pathogenesis of T. vaginalis infection.
Similar articles
-
Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women.Exp Parasitol. 2007 Aug;116(4):399-406. doi: 10.1016/j.exppara.2007.02.007. Epub 2007 Feb 28. Exp Parasitol. 2007. PMID: 17420015
-
A Trichomonas vaginalis cDNA with partial protein sequence homology with a Plasmodium falciparum excreted protein ABRA.Appl Parasitol. 1993 Nov;34(4):245-9. Appl Parasitol. 1993. PMID: 8298655
-
Cysteine proteinase 30 (CP30) and antibody response to CP30 in serum and vaginal washes of symptomatic and asymptomatic Trichomonas vaginalis-infected women.Parasite Immunol. 2007 Jul;29(7):359-65. doi: 10.1111/j.1365-3024.2007.00952.x. Parasite Immunol. 2007. PMID: 17576365
-
[Molecular and chemical mechanism of Trichomonas vaginalis-induced contact-dependent cytotoxicity].Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006 Oct;24(5):375-8. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006. PMID: 17361821 Review. Chinese.
-
Biological roles of cysteine proteinases in the pathogenesis of Trichomonas vaginalis.Parasite. 2014;21:54. doi: 10.1051/parasite/2014054. Epub 2014 Oct 28. Parasite. 2014. PMID: 25348828 Free PMC article. Review.
Cited by
-
Trichomonas vaginalis 62 kDa proteinase as a possible virulence factor.Parasitol Res. 2011 Jan;108(1):241-5. doi: 10.1007/s00436-010-2078-5. Epub 2010 Oct 12. Parasitol Res. 2011. PMID: 20938688
-
Tritrichomonas foetus induces apoptotic cell death in bovine vaginal epithelial cells.Infect Immun. 2004 Jul;72(7):4151-8. doi: 10.1128/IAI.72.7.4151-4158.2004. Infect Immun. 2004. PMID: 15213160 Free PMC article.
-
Clinical and microbiological aspects of Trichomonas vaginalis.Clin Microbiol Rev. 1998 Apr;11(2):300-17. doi: 10.1128/CMR.11.2.300. Clin Microbiol Rev. 1998. PMID: 9564565 Free PMC article. Review.
-
Recent Advances in the Discovery of Novel Antiprotozoal Agents.Molecules. 2019 Oct 28;24(21):3886. doi: 10.3390/molecules24213886. Molecules. 2019. PMID: 31661934 Free PMC article. Review.
-
Mucinases and sialidases: their role in the pathogenesis of sexually transmitted infections in the female genital tract.Sex Transm Infect. 2001 Dec;77(6):402-8. doi: 10.1136/sti.77.6.402. Sex Transm Infect. 2001. PMID: 11714935 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical