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. 1994 Nov 8;91(23):11212-6.
doi: 10.1073/pnas.91.23.11212.

Identification of genes involved in DNA replication of the Autographa californica baculovirus

M Kool  1 , C H Ahrens, R W Goldbach, G F Rohrmann, J M Vlak
Affiliations

Identification of genes involved in DNA replication of the Autographa californica baculovirus

M Kool et al. Proc Natl Acad Sci U S A. .

Abstract

By use of a transient replication assay, nine genes involved in DNA replication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-1, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2, and PE38 stimulate DNA replication. No stimulation by the AcMNPV pcna gene, encoding a protein with sequence homology to proliferating-cell nuclear antigen, was observed. A pattern of amino acids found in a number of single-stranded-DNA-binding proteins was identified in the carboxyl-terminal region of IE-1.

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