[Ultrastructural bases of microcirculatory disorders in intraganglionic capillaries and disorders of nerve elements in the small intestine of suckling rabbits in experimental cholera]

Abstract

The 10-12-day suckling rabbits received culture of V. el tor into the stomach, and the ultrastructural changes of intraganglionic capillaries and nervous elements of small intestine were studied in the course of experimental cholera. It was established that the initial changes 1.5 hrs after the beginning of the experiment were characterized by permeability increase, thrombohemorrhagic syndrome development, oedema of endotheliocytes and perivascular space, fibrin precipitation. There were reactive changes in neurons, but alterations in synapses were absent. In the period of cholera vibrio adhesion (4 hrs) the new signs of endothelial cells desquamation developed. At the same time haemorrhages became more distinct and cells of extravasates more diverse. The development of experimental cholera (1-2 days) was accompanied by appearance of large numbers of coated vesicles in endothelium, tight junctions damage and endothelial cells destruction, extensive dysendothelization which promoted blood cells contact with collagen. In this period dystrophic changes progressed in neurons and activation of cholinergic synapses took place. Enhancement of transepithelial transport of liquid, induced by cholera toxin, is partly connected with activation of cholinergic mechanisms in autonomic ganglia of the small intestine.