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. 1994 Oct 28;204(2):666-72.
doi: 10.1006/bbrc.1994.2511.

Autocrine inhibition of the epidermal growth factor receptor by intracellular expression of a single-chain antibody

R R Beerli  1 , W Wels, N E Hynes
Affiliations

Autocrine inhibition of the epidermal growth factor receptor by intracellular expression of a single-chain antibody

R R Beerli et al. Biochem Biophys Res Commun. .

Abstract

A gene encoding a single-chain antibody which specifically binds the human epidermal growth factor (EGF) receptor has been constructed and expressed intracellularly. The single-chain antibody is derived from monoclonal antibody 225 which competes with EGF for binding to the extracellular domain of the receptor. The single-chain antibody was provided with a signal peptide to direct it to the secretory pathway and was expressed in EGF receptor transformed NIH/3T3 fibroblasts. EGF induced activation of its receptor was reduced in these cells. In addition, EGF-induced anchorage-independent growth of the cells was inhibited. The data suggest that the single-chain antibody functions in an autocrine fashion to inhibit the activity of the EGF receptor.

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