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. 1994 Sep;2(5):411-5.
doi: 10.1007/BF01552801.

Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy

R Martin  1 , W Busch, R G Herrmann, G Wanner
Affiliations

Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy

R Martin et al. Chromosome Res. 1994 Sep.

Abstract

A highly reproducible technique to prepare plant chromosomes for high-resolution field emission scanning electron microscopy is presented. The procedure allows the production of relatively high numbers of chromosome spreads that can be viewed at high resolution, showing structural details below 10 nm. This preparation technique is not restricted to metaphase chromosomes, but also allows the observation of plant chromosomes during all stages of the cell cycle.

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