nmt2 of fission yeast: a second thiamine-repressible gene co-ordinately regulated with nmt1
- PMID: 7992507
- DOI: 10.1002/yea.320100809
nmt2 of fission yeast: a second thiamine-repressible gene co-ordinately regulated with nmt1
Abstract
We previously described a screen for thiamine-repressible genes in Schizosaccharomyces pombe and reported on one such gene, nmt1, required for thiamine biosynthesis. Here we describe a second gene, nmt2, recovered in the same screen. Disruption of nmt2 also resulted in thiamine auxotrophy, indicating a role for the nmt2 gene product in thiamine biosynthesis. Both genes are highly transcribed in minimal medium and repressed in medium containing thiamine, and nuclear 'run-on' experiments confirm that expression in both cases is controlled by the rate of transcription initiation. The virtually identical kinetics of induction and repression suggest that the two genes are co-ordinately regulated. Sequence comparison of the two promoters reveals a canonical TATA box, downstream of which is a perfectly conserved 11 bp element. Transcript mapping experiments show that transcription initiation of both genes is centred on this element.
Similar articles
-
A family of multifunctional thiamine-repressible expression vectors for fission yeast.Yeast. 2000 Jun 30;16(9):861-72. doi: 10.1002/1097-0061(20000630)16:9<861::AID-YEA577>3.0.CO;2-9. Yeast. 2000. PMID: 10861909
-
A chemical compound for controlled expression of nmt1-driven gene in the fission yeast Schizosaccharomyces pombe.Anal Biochem. 2011 May 15;412(2):159-64. doi: 10.1016/j.ab.2011.01.039. Epub 2011 Feb 2. Anal Biochem. 2011. PMID: 21295003
-
nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.J Biol Chem. 1990 Jul 5;265(19):10857-64. J Biol Chem. 1990. PMID: 2358444
-
[Subtractive gene cloning and gene-disruption for elucidation of pseudohyphal formation in Candida tropicalis].Nihon Ishinkin Gakkai Zasshi. 2003;44(2):93-100. doi: 10.3314/jjmm.44.93. Nihon Ishinkin Gakkai Zasshi. 2003. PMID: 12748590 Review. Japanese.
-
Dissecting a complex process.Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2886-7. doi: 10.1073/pnas.91.8.2886. Proc Natl Acad Sci U S A. 1994. PMID: 8159673 Free PMC article. Review. No abstract available.
Cited by
-
The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate.Microb Cell Fact. 2010 Apr 20;9:23. doi: 10.1186/1475-2859-9-23. Microb Cell Fact. 2010. PMID: 20406453 Free PMC article.
-
Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast.EMBO J. 2012 Jan 4;31(1):124-37. doi: 10.1038/emboj.2011.350. Epub 2011 Oct 11. EMBO J. 2012. PMID: 21989386 Free PMC article.
-
Identification of a DNA element in the fission yeast Schizosaccharomyces pombe nmt1 (thi3) promoter involved in thiamine-regulated gene expression.J Bacteriol. 1997 Sep;179(18):5956-8. doi: 10.1128/jb.179.18.5956-5958.1997. J Bacteriol. 1997. PMID: 9294459 Free PMC article.
-
Characterization and expression of the Neurospora crassa nmt-1 gene.Curr Genet. 2003 Dec;44(4):216-23. doi: 10.1007/s00294-003-0441-0. Epub 2003 Sep 17. Curr Genet. 2003. PMID: 13680155
-
Thi1, a thiamine biosynthetic gene in Arabidopsis thaliana, complements bacterial defects in DNA repair.Plant Mol Biol. 1996 Jun;31(3):585-93. doi: 10.1007/BF00042231. Plant Mol Biol. 1996. PMID: 8790291
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases