Isolation of the missing 5'-end of the encoding region of the bovine leukemia virus cell receptor gene

Abstract

The missing 5'-end of the encoding region of the bovine leukemia virus (BLV) cell receptor gene (BLVRcp1/5') was isolated from a lambda gt11 cDNA library using the 32P-labeled EcoRI-SamI fragment corresponding to the 5'-end of a 2.3 kbp cDNA fragment encoding the binding domain of the bovine leukemia virus cell receptor gene (BLVRcp1). The nucleotide and amino acid sequence analysis of the BLVRcp1/5' cDNA revealed that the 1058 bp EcoRI fragment at its 5'-end contained a new 114 amino acid long sequence, and at its 3'-end contained a completely identical 88 amino acid overlapping region with the 5'-end of the BLVRcp1 cDNA. The combined sequences of both cDNAs represent the whole encoding region of the BLV cell receptor gene. The longest open reading frame of the BLV cell receptor gene encodes a protein containing 843 amino acids with a calculated molecular mass of 94.2 kDa which concurs with experimentally detected native BLV receptor protein. Search for homology has shown that about 250 bp of the BLV cell receptor gene is highly homologous to Venter's tag sequences of an unidentified gene from the human brain library.