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. 1994 Nov 15;304 ( Pt 1)(Pt 1):13-6.
doi: 10.1042/bj3040013.

Localization of transglutaminase-reactive glutamine residues in bovine osteopontin

E S Sørensen  1 , L K Rasmussen, L Møller, P H Jensen, P Højrup, T E Petersen
Affiliations

Localization of transglutaminase-reactive glutamine residues in bovine osteopontin

E S Sørensen et al. Biochem J. .

Abstract

Here we report the identification of two transglutaminase-reactive glutamines (Gln-34 and Gln-36) in bovine osteopontin (OPN). Sequence alignment revealed that these glutamines are conserved in all known OPN sequences, indicating a functional importance of this region of the protein. Furthermore, immunological analysis of bovine bone demonstrated that OPN is present in high-molecular-mass complexes in vivo. These findings support the functional aspects of a transglutaminase-catalysed cross-linking of OPN in facilitating cellular attachment and tissue calcification.

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