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. 1994 Nov 30;205(1):361-7.
doi: 10.1006/bbrc.1994.2673.

Western blot analysis of thyrotropin receptor expression in human thyroid tumours and correlation with TSH-binding

E Pötter  1 , R Horn, G F Scheumann, H Dralle, S Costagliola, M Ludgate, G Vassart, J E Dumont, G Brabant
Affiliations

Western blot analysis of thyrotropin receptor expression in human thyroid tumours and correlation with TSH-binding

E Pötter et al. Biochem Biophys Res Commun. .

Abstract

Expression of thyrotropin receptor (TSHr) was analyzed in normal human thyroids and differentiated thyroid tumours by Northern blotting of total RNA and by Western blotting of detergent-solubilized membrane proteins with an antibody specific for the extracellular domain of TSHr. Under nonreducing conditions TSHr stained as a 90-kDa protein in normal thyroid tissue. Expression varied in differentiated carcinomas from normal to not detectable in parallel to steady state TSHr mRNA levels and TSH binding in a TSH binding assay. The negative expression of TSHr in some patients with differentiated carcinomas may have consequences for the TSH suppressive treatments of these patients.

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