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. 1978 Aug 15;173(2):165-71.
doi: 10.1007/BF01851873.

Separation of anti-dinitrophenyl antibodies according to affinity by fractionated elution from immunoadsorbent using thiocyanate

F Shimizu, H Mossmann, K Himmelspach, H Takamiya, A Vogt

Separation of anti-dinitrophenyl antibodies according to affinity by fractionated elution from immunoadsorbent using thiocyanate

F Shimizu et al. Res Exp Med (Berl). .

Abstract

The possibility of fractionating anti-DNP-antibodies according to their affinity by successive elution from a DNP-immunoadsorbent with increasing concentrations of thiocyanate (KSCN) was investigated. Using KSCN at molarities of 0.5 M, 2.0 M and 3.0 M in a stepwise elution procedure, the mean affinity of the eluted antibody fractions was found to increase with increasing molarity of the eluent. The method described is a simple, reliable means of separating antibodies on an affinity basis which is of special value in systems where haptens are not available.

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