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. 1976 Sep;31(3):337-41.

Isolation of human eosinophils and their lymphocyte-like rosetting properties

R Sher, A Glover

Isolation of human eosinophils and their lymphocyte-like rosetting properties

R Sher et al. Immunology. 1976 Sep.

Abstract

A method for the isolation of large numbers of almost pure human eosinophils from peripheral blood using hypague-ficoll density gradients, gelatin sedimentation and carbonyl iron is described. Eosinophils were obtained from patients with parasitic infections, allergic reactions, undiagnosed eosinophilias and normal volunteers. Erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosettes were formed by a number of eosinophils.

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References

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