Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Mar;38(3):205-10.
doi: 10.1007/BF00176082.

Single-strand regions of poly(G) act as templates for oligo(C) synthesis

H Rembold  1 , L E Orgel
Collaborators, Affiliations

Single-strand regions of poly(G) act as templates for oligo(C) synthesis

H Rembold et al. J Mol Evol. 1994 Mar.

Abstract

Oligonucleotide primers consisting of a sequence of four or more deoxycytidylate residues terminated by a single ribocytidylate residue are extended by reaction with cytidine 5'-phosphoro(2-methyl)imidazolide using polyguanylic acid as a template. The efficiency of the reaction decreases as the length of the primer increases. The reaction does not seem to depend on the dissociation of poly(G) tetrahelices but uses as templates single-stranded segments that are already present in enzymatically synthesized polyguanylic acid.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Nature. 1990 Mar 29;344(6265):410-4 - PubMed
    1. J Am Chem Soc. 1992;114(14):5496-501 - PubMed
    1. Biopolymers. 1977 Apr;16(4):791-809 - PubMed
    1. Science. 1983 Feb 18;219(4586):859-62 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1987;52:41-51 - PubMed

Publication types

LinkOut - more resources