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. 1994 May;29(5):365-8.
doi: 10.1007/BF02537191.

A rapid method for the preparation of ganglioside Glac2 (GD3)

R Jennemann  1 , H Wiegandt
Affiliations

A rapid method for the preparation of ganglioside Glac2 (GD3)

R Jennemann et al. Lipids. 1994 May.

Abstract

A method is described for the preparation of ganglioside Glac2 [(II3(NeuAc)2-LacCer, GD3] from cream of bovine milk using liquid-phase extraction with methanol or ethanol followed by anion exchange chromatography. The method is rapid and inexpensive; 1 kg cream, centrifuged from 14-15 L of bovine milk, yields approximately 70 mg of pure ganglioside Glac2. The sialic acid constituent of ganglioside Glac2 isolated from bovine milk cream consists solely of the N-acetylneuraminic acid derivative. The major components of its ceramide consist of octadecasphing-4-enine and the 22:0 (behenic acid) and 23:0 fatty acids.

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References

    1. Chem Phys Lipids. 1975 Nov;15(2):105-24 - PubMed
    1. Biochim Biophys Acta. 1972 Oct 5;280(2):356-64 - PubMed
    1. Biochim Biophys Acta. 1973 Jan 19;296(1):160-70 - PubMed
    1. J Biol Chem. 1982 Nov 10;257(21):12752-6 - PubMed
    1. Z Naturforsch B. 1964 Mar;19:256-7 - PubMed

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