doi: 10.1002/yea.320100309.
Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae
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- PMID: 8017106
- DOI: 10.1002/yea.320100309
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Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae
Yeast.
1994 Mar.
Abstract
We have cloned NES24 using a temperature-sensitive nes24-1 mutant as a host and sequenced a 3162 bp XhoI-EcoRI DNA fragment containing the NES24 gene. Computer analysis revealed that this segment contains a 1806 bp open reading frame which is needed for complementation of the nes24-1 mutation. We found SUP8 in the region upstream of the NES24 gene, placing the NES24 gene on chromosome XIII. A protein homology search indicated that NES24 encodes a new protein. The disruption of the NES24 gene resulted in temperature-sensitive growth. The sequence has been deposited in DDBJ/EmBL/GenBank data bases under Accession Number D15052.
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