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. 1994 Mar;10(3):371-6.
doi: 10.1002/yea.320100309.

Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae

M Yoshida  1 , Y Shimma, I Uno, A Toh-e
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Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae

M Yoshida et al. Yeast. 1994 Mar.

Abstract

We have cloned NES24 using a temperature-sensitive nes24-1 mutant as a host and sequenced a 3162 bp XhoI-EcoRI DNA fragment containing the NES24 gene. Computer analysis revealed that this segment contains a 1806 bp open reading frame which is needed for complementation of the nes24-1 mutation. We found SUP8 in the region upstream of the NES24 gene, placing the NES24 gene on chromosome XIII. A protein homology search indicated that NES24 encodes a new protein. The disruption of the NES24 gene resulted in temperature-sensitive growth. The sequence has been deposited in DDBJ/EmBL/GenBank data bases under Accession Number D15052.

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