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. 1994 Apr;59(4):485-93.

[A new site-specific endonuclease BspKT51]

[Article in Russian]
N I Shapovalova, L A Zheleznaia, N N Matvienko, N I Matvienko
  • PMID: 8018770

[A new site-specific endonuclease BspKT51]

[Article in Russian]
N I Shapovalova et al. Biokhimiia. 1994 Apr.

Abstract

The new site-specific endonuclease BspKT5I free from interfering impurities has been isolated from thermophilic soil bacteria Bacillus species KT5 by successive chromatography on blue agarose, hydroxyapatite and heparin-Sepharose. BspKT5I on double-stranded DNA recognizes the sequence 5'-CTGAAG16N decreases 3'-GACTTC14N increases and cleaves the DNA at the recognition site as indicated by the arrows to form dinucleotide 3'-protruding termini. The isolated endonuclease is an isoschisomer of Eco57I. However, unlike Eco57I, it is not stimulated by S-adenosylmethionine (SAM) and can therefore be related to subclass IIs but not to IV, as Eco57I. In addition, endonuclease BspKT5I, unlike Eco57I, has no methylase activity.

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