Location of PHM/VIP mRNA in human gastrointestinal tract detected by in situ hybridization
- PMID: 8020060
- DOI: 10.1007/BF00306108
Location of PHM/VIP mRNA in human gastrointestinal tract detected by in situ hybridization
Abstract
The expression of the gene for vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM) in the human gastrointestinal tract was studied by in situ hybridization and Northern blotting for PHM/VIP mRNA and immunocytochemistry using specific antisera against the bioactive peptides PHM and VIP. In the colon sigmoideum, antisera against all five putative processing products of the VIP precursor (prepro-VIP) were used, namely prepro-VIP 22-79, PHM, prepro-VIP 111-122, VIP and prepro-VIP 156-170. Furthermore, RNA extracted from various regions of the gastrointestinal tract was examined by Northern blots and hybridization to a VIP-cDNA probe. Throughout the gastrointestinal tract, PHM/VIP mRNA was found in neurons only. Using single- or double-staining methods, we demonstrated both PHM/VIP mRNA and the corresponding peptides PHM and VIP in the neurons. In the sigmoideum, the single-staining methods were extended to investigate whether the neurons simultaneously contained PHM/VIP mRNA and each of the five prepro-VIP-derived peptides. Only one major band of PHM/VIP mRNA (1.9 kb) was found by Northern blotting in the tissue of the gastrointestinal tract.
Similar articles
-
Gene expression and transcript size of the prepro-peptide VIP/PHM-27 in normal human tissue.Neurosci Lett. 1993 Dec 24;164(1-2):101-4. doi: 10.1016/0304-3940(93)90867-k. Neurosci Lett. 1993. PMID: 8152582
-
Characterization and regional distribution of peptides derived from the vasoactive intestinal peptide precursor in the normal human brain.J Neurochem. 1989 Oct;53(4):1142-8. doi: 10.1111/j.1471-4159.1989.tb07407.x. J Neurochem. 1989. PMID: 2769258
-
Cyclic AMP regulation of pro-vasoactive intestinal polypeptide/PHM-27 synthesis in human neuroblastoma cells.J Biol Chem. 1984 Jul 25;259(14):9207-11. J Biol Chem. 1984. PMID: 6086627
-
Concentrations and distribution of vasoactive intestinal peptide (VIP), peptide histidine isoleucine (PHI) and peptide histidine valine (PHV) in the cerebral cortex and the suprachiasmatic nucleus of the mouse.Brain Res. 1994 Sep 5;656(1):95-107. doi: 10.1016/0006-8993(94)91370-6. Brain Res. 1994. PMID: 7804850
-
Biosynthesis and Function of VIP and Oxytocin: Mechanisms of C-terminal Amidation, Oxytocin Secretion and Transport.Endocrinology. 2023 Aug 1;164(9):bqad121. doi: 10.1210/endocr/bqad121. Endocrinology. 2023. PMID: 37548257 Review.
Cited by
-
Functional coupling between nitric oxide synthesis and VIP release within enteric nerve terminals of the rat: involvement of protein kinase G and phosphodiesterase 5.J Physiol. 2001 Aug 1;534(Pt 3):827-36. doi: 10.1111/j.1469-7793.2001.00827.x. J Physiol. 2001. PMID: 11483712 Free PMC article.
-
The topography, architecture and structure of the enteric nervous system in the jejunum and ileum of cattle.J Anat. 1999 Jul;195 ( Pt 1)(Pt 1):1-9. doi: 10.1046/j.1469-7580.1999.19510001.x. J Anat. 1999. PMID: 10473287 Free PMC article.
-
Regulation of VIP gene expression in general. Human lung cancer cells in particular.J Mol Neurosci. 1996 Summer;7(2):99-110. doi: 10.1007/BF02736790. J Mol Neurosci. 1996. PMID: 8873894 Review.