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Comparative Study
. 1994 May 15;118(3):233-6.
doi: 10.1111/j.1574-6968.1994.tb06833.x.

Comparative development of Cryptosporidium parvum (Apicomplexa) in 11 continuous host cell lines

S J Upton  1 , M Tilley, D B Brillhart
Affiliations
Comparative Study

Comparative development of Cryptosporidium parvum (Apicomplexa) in 11 continuous host cell lines

S J Upton et al. FEMS Microbiol Lett. .

Abstract

Using standardized media, incubation, and parasite inoculating procedures, we compared development of Cryptosporidium parvum between Madin-Darby bovine kidney (MDBK) cells and 10 additional host cell lines available through the American Type Culture Collection. Parasite development was assessed by counting parasite numbers atop monolayers in 25 random oil fields 68 h post-infection using Nomarski interference-contrast optics. Results revealed that the human ileocecal adenocarcinoma (HCT-8) cell line supported nearly twice the number of parasite developmental stages than MDBK cells or any of the other host cell types.

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