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Comparative Study
. 1994 Apr;32(4):1054-7.
doi: 10.1128/jcm.32.4.1054-1057.1994.

Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab

R M Wadowsky  1 , S Laus, T Libert, S J States, G D Ehrlich
Affiliations
Comparative Study

Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab

R M Wadowsky et al. J Clin Microbiol. 1994 Apr.

Abstract

A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.

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