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Comparative Study
. 1994 Apr;32(4):987-90.
doi: 10.1128/jcm.32.4.987-990.1994.

Neutralization enzyme immunoassay for influenza virus

C A Benne  1 , M Harmsen, J C De Jong, C A Kraaijeveld
Affiliations
Comparative Study

Neutralization enzyme immunoassay for influenza virus

C A Benne et al. J Clin Microbiol. 1994 Apr.

Abstract

A neutralization enzyme immunoassay (N-EIA) was developed for the detection of antibody titer rises in sera of patients infected with influenza A (H3N2) virus. In this N-EIA, a selected strain of influenza A (H3N2) virus was added to monolayers of LLC-MK2 cells in microtiter plates. After 24 h, the replicated virus could be demonstrated with a virus-specific enzyme-labeled monoclonal antibody. Preincubation of the influenza virus with convalescent-phase sera of patients infected with influenza A (H3N2) virus resulted 1 day later in decreased absorbance values that could be used for calculation of neutralization titers. From use of paired serum samples from 10 patients with a history of flu-like symptoms, the results obtained with N-EIA correlated well (r = 0.83) with those of the standard hemagglutination inhibition test.

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