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. 1975 Jan;191(1):127-31.
doi: 10.1002/jez.1401910113.

Immunofluorescent localization of bovine acrosin

D L Garner, M P Easton, M E Munson, M A Doane

Immunofluorescent localization of bovine acrosin

D L Garner et al. J Exp Zool. 1975 Jan.

Abstract

Acrosin, a trypsin-like proteinase, was localized in the acrosome of methanol-fixed bovine spermatoza by the indirect immunofluorescent technique. Anti-acrosin immunoglobulin was obtained from a rabbit immunized with highly purified bovine acrosin. The results of agarose-gel immunnodiffusion analyses indicated that although the antibody was specific for the acrosomal enzyme, it cross-related with ovine acrosin.

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