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. 1975 Feb;64(2):331-9.
doi: 10.1083/jcb.64.2.331.

Two improved methods for preparing ferritin-protein conjugates for electron microscopy

Y Kishida, B R Olsen, R A Berg, D J Prockop

Two improved methods for preparing ferritin-protein conjugates for electron microscopy

Y Kishida et al. J Cell Biol. 1975 Feb.

Abstract

Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.

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