Cloning of Entamoeba genes encoding proteolipids of putative vacuolar proton-translocating ATPases
- PMID: 8039932
- PMCID: PMC302996
- DOI: 10.1128/iai.62.8.3572-3575.1994
Cloning of Entamoeba genes encoding proteolipids of putative vacuolar proton-translocating ATPases
Abstract
Molecular cloning techniques were used to identify genes encoding the proteolipids of putative vacuolar proton-transporting ATPases (V-ATPases; EC 3.6.1.35) of Entamoeba histolytica (Ehvma3) and Entamoeba dispar. The Ehvma3 gene encoded a 177-amino-acid peptide, with an M(r) of 18,110, which showed extensive positional identities with peptides of E. dispar (92%), Schizosaccharomyces pombe (58%), and humans (56%).
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