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Comparative Study
. 1994 Jul 11;22(13):2460-5.
doi: 10.1093/nar/22.13.2460.

Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

E Nordhoff  1 , F Kirpekar, M Karas, R Cramer, S Hahner, F Hillenkamp, K Kristiansen, P Roepstroff, A Lezius
Affiliations
Free PMC article
Comparative Study

Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

E Nordhoff et al. Nucleic Acids Res. .
Free PMC article

Abstract

UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

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