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. 1994;161(5):439-41.
doi: 10.1007/BF00288956.

Restriction endonucleases from Selenomonas ruminantium which recognize and cleave 5'-AT/TAAT-3'

P Pristas  1 , I Vanat, A Godany, P Javorský
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Restriction endonucleases from Selenomonas ruminantium which recognize and cleave 5'-AT/TAAT-3'

P Pristas et al. Arch Microbiol. 1994.

Abstract

Two natural isolates from fallow-deer rumen identified as Selenomonas ruminantium were found to produce a restriction endonuclease which we called Sru4DI. This enzyme was isolated from cell extracts by phosphocellulose chromatography. Analysis of the Sru4DI recognition site showed that Sru4DI recognizes the hexanucleotide sequence 5'-AT/TAAT-3' generating 5'dinucleotide protruding ends upon cleavage and thus is a true isoschizomer of vspI, a restriction enzyme isolated from Vibrio sp.

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References

    1. Gene. 1992 Feb 1;111(1):105-8 - PubMed
    1. Appl Environ Microbiol. 1992 Jan;58(1):66-9 - PubMed
    1. Methods Enzymol. 1980;65(1):391-404 - PubMed
    1. Lett Appl Microbiol. 1993 Dec;17(6):297-9 - PubMed
    1. J Bacteriol. 1992 Aug;174(16):5275-83 - PubMed

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