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. 1994 Jul;13(1):167-77.
doi: 10.1016/0896-6273(94)90467-7.

Depolarization-transcription signals in skeletal muscle use calcium flux through L channels, but bypass the sarcoplasmic reticulum

C F Huang  1 , B E Flucher, M M Schmidt, S K Stroud, J Schmidt
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Depolarization-transcription signals in skeletal muscle use calcium flux through L channels, but bypass the sarcoplasmic reticulum

C F Huang et al. Neuron. 1994 Jul.

Retraction in

Abstract

Membrane depolarization inactivates acetylcholine receptor (AChR) genes in skeletal muscle. We have studied this process in C2C12 cells, focusing on the role of calcium. Cytoplasmic calcium was monitored with fluo-3, and the activity of receptor genes was measured with a sensitive transcript elongation assay. Removal of extracellular calcium or blockage of L-type calcium channels disrupts signaling, even when release of calcium from the sarcoplasmic reticulum (SR) is not impeded, whereas L channel agonists induce signaling without membrane depolarization or release of calcium from intracellular stores. Activators of calcium release from the SR do not inhibit AChR genes, either in C2C12 or in chicken skeletal muscle in vivo. It appears that calcium ions do not act as messengers between sarcolemma and nucleus but target a sensor near their port of entry where they initiate a signal that bypasses the SR.

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  • Findings of scientific misconduct.
    [No authors listed] [No authors listed] NIH Guide Grants Contracts (Bethesda). 1999 May 11:1 p. NIH Guide Grants Contracts (Bethesda). 1999. PMID: 12458592 Free PMC article. No abstract available.

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