Distribution of indigenous bacteria in the digestive tract of conventional and gnotobiotic rats

Abstract

The localization and population levels of the indigenous bacterial flora of conventional rats were investigated by cultural and histological techniques. Lactobacilli predominate in the stomach and upper part of the small intestine and associate with keratinized cells of the nonglandular portion of stomach. Mixtures of varying complexity of pure cultures of indigenous bacteria were inoculated into germfree rats. The distribution of these bacteria was examined to investigate the effect of lactobacilli in controlling the composition of other bacterial species in each portion of the digestive tract. In the stomach and the upper part of the small intestine, lactobacilli controlled the population levels of other bacterial species. In the lower part of the small intestine, not only lactobacilli but also the anaerobes which colonized the large bowel influenced the population levels of other bacterial types. Staphylococci isolated from a conventional rat colonized specifically the keratinized cells of the nonsecreting epithelium of the stomach when the rats were free from lactobacilli. This colonization was not observed after inoculation of lactobacilli into the rats.