Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Aug;126(3):591-601.
doi: 10.1083/jcb.126.3.591.

Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly

S Dimitrov  1 , M C Dasso, A P Wolffe
Affiliations

Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly

S Dimitrov et al. J Cell Biol. 1994 Aug.

Abstract

We find that the remodeling of the condensed Xenopus laevis sperm nucleus into the paternal pronucleus in egg extracts is associated with phosphorylation of the core histones H2A, H2A.X and H4, and uptake of a linker histone B4 and a HMG 2 protein. Histone B4 is required for the assembly of chromatosome structures in the pronucleus. However neither B4 nor core histone phosphorylation are required for the assembly of spaced nucleosomal arrays. We suggest that the spacing of nucleosomal arrays is determined by interaction between adjacent histone octamers under physiological assembly conditions.

PubMed Disclaimer

References

    1. Biochemistry. 1979 Aug 21;18(17):3739-48 - PubMed
    1. FEBS Lett. 1980 Oct 6;119(2):337-42 - PubMed
    1. Cell Differ. 1980 Dec;9(6):339-50 - PubMed
    1. Nucleic Acids Res. 1980 Oct 24;8(20):4671-80 - PubMed
    1. Dev Biol. 1981 May;84(1):79-87 - PubMed