[Histochemical study of sarcoid granulomatous lesions]

Abstract

Immunohistochemical study of sarcoid granulomatous lesions. In first we studied the distribution of each functionally distinct T lymphocyte subsets in sarcoid lesions by use of a single and double stain methods with monoclonal antibodies for T cell markers including CD4, CD8, CD45RA, and CD45RO. The results showed that CD4(+)+CD45RA- T cells "Helper/helper inducer" distributed diffusely though the lesions and CD8(+)+CD45RA- T cells "Cytotoxic" distributed at edge of the granulomas. And CD4(+)+CD45RA+ T cells "Suppressor inducer" and CD8(+)+CD45RA+ T cells "Suppressor" also distributed near the granulomas. In second we studied populations of T cells expressed with each antigen including CD26, 28, 29, 11a, 54, 43, 58, 69, 45RA, 45RO, and CD25. The results showed that T cells expressed with each antigen as described above were more numerously in granulomas than out of granulomas. In third we studied T cells designated as T helper 1 (Th-1) and T helper 2 (Th-2) by use of ant-IFN gamma for Th-1 and anti-IL4 and IL6 for Th-2. The results showed that IFN gamma producing cells were scantily demonstrated out of granulomas and conversely IL4 and IL6 producing cells were demonstrated more numerously in granulomas than out of granulomas. From above results we concluded that Immunologic event in sarcoidosis might be Th-2 biased response.