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. 1975 Apr 22;14(8):1643-7.
doi: 10.1021/bi00679a016.

Characterization of polypyrimidines in Drosophila and L-cell DNA

Characterization of polypyrimidines in Drosophila and L-cell DNA

H C Birnboim et al. Biochemistry. .

Abstract

Unusually long pyrimidine tracts (polypyrimidines), ranging from 100 to over 1000 nucleotides in length, have been found in Drosophila melanogaster DNA. They are compared to shorter pyrimidine tracts (25-150 nucleotides) which have previously been found in L-cell DNA. Both species were able to anneal to homologous DNA; Drosophila polypyrimidines formed stable hybrids while L-cell polypyrimidines formed hybrids of lower thermal stability. In both cases, the kinetics of the reaction was rapid, suggesting that these tracts are part of highly repeated DNA.

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