The role of divalent cations in the reactions of valyl transfer ribonucleic acid synthetase of Escherichia coli. Effects of spermine and ethylenediaminetetraacetate
- PMID: 805132
Item in Clipboard
The role of divalent cations in the reactions of valyl transfer ribonucleic acid synthetase of Escherichia coli. Effects of spermine and ethylenediaminetetraacetate
J Biol Chem.
.
Free article
Abstract
We have analyzed the function of spermine in the aminoacylation of tRNA-Val by the valyl-tRNA synthetase of Escherichia coli. Our results indicate that Mg2+ is required for the aminoacylation reaction as well as for the ATP-PP-i exchange catalyzed by this enzyme. The apparent stimulation by spermine is a function of the tRNA used, which appears to contain bound cations even after dialysis against 10 minus 4 M EDTA. Higher concentrations of EDTA totally abolish spermine-stimulated esterification of tRNA-Val.
Similar articles
-
Aminoacyl transfer RNA formation. V. Effect of ethylenediaminetetraacetate on isoleucyl transfer RNA formation stimulated by either spermine or Mg2+.J Biol Chem. 1975 May 25;250(10):3878-82. J Biol Chem. 1975. PMID: 805133
-
The role of polyamines in the aminoacyl transfer ribonucleic acid synthetase reactions. Demonstration of the requirement for magnesium ion and a secondary stimulatory effect of spermine.J Biol Chem. 1975 May 25;250(10):3874-7. J Biol Chem. 1975. PMID: 165187
-
Mechanism of aminoacylation of tRNA. Influence of spermine on the kinetics of aminoacyl-tRNA synthetases by isoleucyl- and valyl-tRNA synthetases from Mycobacterium smegmatis.Biochim Biophys Acta. 1981 Jun 26;654(1):94-101. doi: 10.1016/0005-2787(81)90140-4. Biochim Biophys Acta. 1981. PMID: 6912073
-
Yellow lupin (Lupinus luteus) aminoacyl-tRNA synthetases. Isolation and some properties of enzyme-bound valyl adenylate and seryl adenylate.Biochim Biophys Acta. 1978 Dec 21;521(2):584-96. doi: 10.1016/0005-2787(78)90300-3. Biochim Biophys Acta. 1978. PMID: 32907
-
[Superspecificity of aminoacyl-tRNA-synthases].Mol Biol (Mosk). 1984 Jan-Feb;18(1):205-26. Mol Biol (Mosk). 1984. PMID: 6423966 Review. Russian.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
