Aminoacyl transfer RNA formation. V. Effect of ethylenediaminetetraacetate on isoleucyl transfer RNA formation stimulated by either spermine or Mg2+

Abstract

EDTA inhibited isoleucyl tRNA formation stimulated by spermine in Escherichia coli. It was found that the inhibition by EDTA was not due to contamination of the constituents of the reaction mixture with Mg2+ or to inhibition of the binding of spermine to tRNA by EDTA, but to the interaction of EDTA with isoleucyl-tRNA synthetase. These data suggest the isoleucyl-tRNA synthetase of E. coli contains some metal ion, which is necessary for the enzyme to catalyze the reaction.