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. 1994;11(1):7-11.
doi: 10.1016/1050-3862(94)90003-5.

High-throughput rapid yeast DNA extraction. Application to yeast artificial chromosomes as polymerase chain reaction templates

M M Blanchard  1 , V Nowotny
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High-throughput rapid yeast DNA extraction. Application to yeast artificial chromosomes as polymerase chain reaction templates

M M Blanchard et al. Genet Anal Tech Appl. 1994.

Abstract

An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4 degrees C is active in polymerase chain reaction experiments for more than a year.

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