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. 1994 Aug 15;302 ( Pt 1)(Pt 1):11-4.
doi: 10.1042/bj3020011.

Cloning of cDNA encoding the bifunctional dehydroquinase.shikimate dehydrogenase of aromatic-amino-acid biosynthesis in Nicotiana tabacum

C A Bonner  1 , R A Jensen
Affiliations

Cloning of cDNA encoding the bifunctional dehydroquinase.shikimate dehydrogenase of aromatic-amino-acid biosynthesis in Nicotiana tabacum

C A Bonner et al. Biochem J. .

Abstract

Nicotiana tabacum cDNA encoding a bifunctional protein having catalytic domains for dehydroquinase and shikimate dehydrogenase was cloned and sequenced. Complementation of Escherichia coli aroD and aroE auxotrophs was successful. Amino acid sequencing located the N-terminus of the mature protein. The two catalytic domains exhibited greater amino acid identity with prokaryote homologues than with yeast and fungal homologues.

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References

    1. J Bacteriol. 1966 Apr;91(4):1494-508 - PubMed
    1. Mol Microbiol. 1993 Oct;10(2):385-95 - PubMed
    1. J Mol Biol. 1986 Jan 5;187(1):101-7 - PubMed
    1. Nucleic Acids Res. 1986 Mar 11;14(5):2201-13 - PubMed
    1. Biochem J. 1986 Sep 1;238(2):475-83 - PubMed

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