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. 1994 Apr;17(4):539-42.
doi: 10.1248/bpb.17.539.

Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake)

A Hirata  1 , Y Adachi, W Itoh, M Komoda, K Tabata, I Sugawara
Affiliations
Free article

Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake)

A Hirata et al. Biol Pharm Bull. 1994 Apr.
Free article

Abstract

A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) showed that MAb MPG2 reacted with lysosomal proteoglycan and (1-->6)-beta-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1-->3)-beta-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.

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