Polysialic acid of the neural cell adhesion molecule (N-CAM) is widely expressed during organogenesis in mesodermal and endodermal derivatives

Abstract

We have studied the expression of homopolymers of alpha 2,8-linked sialic acid (polySia) and the neural cell adhesion molecule (N-CAM) during the embryonic and fetal development of rat, chicken and man using immunocytochemistry and immunoblotting. During development, polySia and N-CAM were widely expressed in mesodermally and neuro-ectodermally derived elements. In specific developmental processes, cells of endodermal and ectodermal (non-neural) origin were also immunoreactive for these molecules. Loss of polySia and N-CAM immunoreactivity often accompanied differentiation of mesodermally derived cells. In cartilage formation for instance, cells in precartilaginous mesenchymal condensations stained for N-CAM and polySia until the first appearance of specific chondrocyte function, independent of the stage of development. Transient de novo expression of polySia, in newly induced ectodermal cells, paralleled the reciprocal inductive interactions of mesodermally derived cells with cells of ectodermal origin during hair follicle formation. All ectodermally derived hair follicle cells, except the putative stem cells, later ceased expression of these molecules. Ectodermal expression of polySia and N-CAM was otherwise rare. The endodermally derived epithelium of the digestive and respiratory tracts were polySia and N-CAM immunoreactive early in organogenesis (embryonic day 12 in mouse). Cells of this derivation later all became unreactive, although decrease in immunoreactivity during development was faster in derivatives of more cranial portions of the endoderm. In general, during organogenesis, epithelial elements showed polySia and N-CAM expression before and during epithelium formation, thereafter losing immunoreactivity, irrespective of the developmental origin of the epithelial cells. PolySia and N-CAM staining in the chicken respiratory tract epithelium was more wide-spread and lasted significantly longer than in either man or rat. Cells that expressed N-CAM, but not polySia, were found during the development of both skin and pancreas, indicating independent control of polySia expression. Outside the nervous system no cells that expressed polySia but not N-CAM were observed.